| Applications: | ELISA, WB, FACS, ICC/IF |
| Species Reactivity: | Human |
| Isotype: | Mouse IgG2b kappa |
| Immunogen: | Recombinant human CD73 (27-252aa) purified from E. coli |
| Purification: | By protein-G affinity chromatography |
| Conjugation: | Unconjugated |
| Description: | CD73 (Ecto-5-nucleotidase, 5-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells. |
| Further Information: | The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000. |
| Synonym: | 5' nucleotidase, ecto, 5'-nucleotidase ecto (CD73), 5' nucleotidase (CD73), CD73, eN, eNT |
| Ascension Number: | P21589 |
| NCBI EntrezGene: | NP_002517 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
