Ordering Details
Cat Number:	ACB0825-50ul
Size:	50uL
Concentration:	1mg/mL
Price:	£123.00
Quantity:
Shipping:	£12.00

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Cyclophilin B [k2E2], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, FACS, ICC/IF
Species Reactivity:	Human
Isotype:	Mouse IgG1 kappa
Immunogen:	Recombinant human Cyclophilin B (26-216aa) purified from E. coli
Purification:	By protein-G affinity chromatography
Conjugation:	Unconjugated
Description:	Cyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Cyclophilin B is necessary for the prolactin-induced proliferation, cell growth, and the nuclear retrotransport of prolactin.
Further Information:	The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.
Synonym:	PPIB, Peptidylprolyl isomerase B, CYPB, SCYLP, CYP-S1: Cyclophilin-B, Cyclophilin B, 26- 216aa, AA553318, AI844835, Cphn 2, Cphn2, CyP 20b, AA408962, CYP S1, MGC14109, MGC2224, peptidyl prolyl cis trans isomerase B, Peptidyl prolyl cis trans isomerase B precursor, PPIase, Rotamase, S cyclophilin.
Ascension Number:	P23284
NCBI EntrezGene:	NP_000933
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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Cell lysates of HepG2(30ug) and HeLa(30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Cyclophilin B (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclophilin B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Jurkat cell lysateLane 2.: K562 cell lysateLane 3.: 293T cell lysateLane 4.: A549 cell lysateLane 5.: MCF7 cell lysateLane 6.: SK-OV-3 cell lysateLane 7.: LnCap cell lysateThe Recombinant Protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclophilin B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Cyclophilin B Recombinant ProteinLane 2.: Cyclophilin A Recombinant ProteinLane 3.: Cyclophilin D Recombinant ProteinLane 4.: Cyclophilin E Recombinant ProteinLane 5.: Cyclophilin F Recombinant ProteinLane 6.: Cyclophilin G Recombinant ProteinLane 7.: Cyclophilin H Recombinant Protein

ICC/IF analysis of Cyclophilin B in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Cyclophilin B antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).