Recombinant crystallin alpha A (alphaA) and the extract of mouse eye (ME) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human crystallin alpha B antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
ICC/IF analysis of Crystallin alpha B in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Crystallin alpha B antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).