| Applications: | WB, IF, IP |
| Dilutions: | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000). |
| Isotype: | Mouse IgG |
| Immunogen: | Synthetic Peptide |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
| Description: | Featured Anti-T7 Tag Mouse Monoclonal Antibody (6D4), specially designed for your taged protein quantification and localization. |
| Further Information: | T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10, which encodes a T7 major capsid protein. The T7 tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems. |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Stable for one year at -20 C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Usage: | Research use only |
