Applications: | WB, IF, IP |
Dilutions: | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000). |
Isotype: | Mouse IgG |
Immunogen: | Synthetic Peptide |
Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
Description: | Featured Anti-KT3 Tag Mouse Monoclonal Antibody (14D8), specially designed for your taged protein quantification and localization. |
Further Information: | The KT3 epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. KT3 epitope tag can be used to monitor expression of the protein products transfected with cDNA constructs. The recognized KT3 epitope represents the amino acid sequence KPPTPPPEPET derived from the Simian Virus 40 (SV40) large T-antigen. |
Shipping Conditions: | Ship on cold packs |
Storage: | Stable for one year at -20 C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
Usage: | Research use only |