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Cat Number:ABT2051-2
Size:200uL
Price:£229.00
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Shipping:£12.00

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His Tag, Rabbit Polyclonal Antibody

Partner: Abbkine Scientific

Applications:WB, IF, IP
Dilutions:Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000).
Isotype:Rabbit IgG
Immunogen:Synthetic Peptide
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen
Description:Featured Anti-His Tag Rabbit Polyclonal Antibody, specially designed for your taged protein quantification and localization.
Further Information:A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
Shipping Conditions:Ship on cold packs
Storage:Stable for one year at -20 C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Usage:Research use only

 

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A02051-wb.jpg&&Fig. Western blot analysis of His fusion protein in cell lysate from transfected 293T cells (lane A) and baculovirus expression vector system (lane B) at 1:2000 dilutions.

A02051-wb.jpg&&Fig. Western blot analysis of His fusion protein in cell lysate from transfected 293T cells (lane A) and baculovirus expression vector system (lane B) at 1:2000 dilutions.