| Applications: | WB, IF, IP |
| Dilutions: | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000), IF (1:1500), IP (1:300). |
| Isotype: | Mouse IgG |
| Immunogen: | Synthetic Peptide |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
| Description: | Featured Anti-His Tag Mouse Monoclonal Antibody (5C3), specially designed for your taged protein quantification and localization. |
| Further Information: | A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems. |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Stable for one year at -20 C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Usage: | Research use only |
