A02010-if.jpg&&Fig.1. Immunofluorescence staining (1:4000) of Flag fusion protein in 293 cells and counterstained with DAPI.|||A02010-wb.jpg&&Fig.2. IP (1:400)-WB (1:10000) analysis of Flag fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with Flag-tag protein (lane B), IP transfected 293 with normal Mouse IgG and Protein G agarose (lane C), IP transfected 293 with Anti Flag tag mAb and Protein G agarose (lane D), and IP transfected 293 with only Protein G agarose (lane E).