Ordering Details
Cat Number:	ABL1080-2
Size:	200uL
Price:	£229.00
Quantity:
Shipping:	£12.00

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alpha-Tubulin [3G5], Mouse Monoclonal Antibody

Partner: Abbkine Scientific

Applications:	WB, IHC, IF, IP
Species Reactivity:	Human, Mouse, Rat
Dilutions:	Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000-1:10000), IHC-P (1:50-1:300), IF (1:200), IP (1:200).
Isotype:	Mouse IgG
Immunogen:	Recombinant Protein
Purification:	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Description:	Featured Anti-alpha-Tubulin Monoclonal Antibody (3G5), specially designed for your immunoassay as internal control or location staining.
Further Information:	Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. TUBA1A encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that TUBA1A expression is predominantly found in morphologically differentiated neurologic cells. TUBA1A is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in TUBA1A cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, mental retardation, and early-onset epilepsy and caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
NCBI EntrezGene:	7846/10376
Shipping Conditions:	Ship on cold packs
Storage:	Stable for one year at -20 C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Usage:	Research use only

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A01080-1.jpg&&Fig.1. Western blot analysis of 1) Hela, 2) rat brian tissue, 3) mouse brain tissue, diluted at 1:5000.|||A01080-2.jpg&&Fig.2. Immunofluorescence analysis of human colon cancer tissue. 1, alpha-tubulin Monoclonal Antibody (3G5) (red) was diluted at 1:200 (4�C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.|||A01080-3.jpg&&Fig.3. Immunofluorescence analysis of mouse liver tissue. 1, alpha-tubulin Monoclonal Antibody (3G5) (red) was diluted at 1:200 (4�C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.|||A01080-4.jpg&&Fig.4. Immunohistochemical analysis of paraffin-embedded human uterus cancer tissue. 1, alpha-tubulin Monoclonal Antibody (3G5) was diluted at 1:200 (4�C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98�C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.|||A01080-5.jpg&&Fig.5. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, alpha-tubulin Monoclonal Antibody (3G5) was diluted at 1:200 (4�C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98�C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.|||A01080-6.jpg&&Fig.6. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, alpha-tubulin Monoclonal Antibody (3G5) was diluted at 1:200 (4�C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98�C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.