Ordering Details
Cat Number:	ABL1070-2
Size:	200uL
Price:	£109.00
Quantity:
Shipping:	£12.00

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Histone H3 [2D10], Mouse Monoclonal Antibody

Partner: Abbkine Scientific

Applications:	WB, IHC, IF, IP
Species Reactivity:	Human, Mouse, Rat, Yeast, Rice
Dilutions:	Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:2000-1:5000), IHC-P (1:50-1:300), IF (1:100-1:500), IP (1:200).
Isotype:	Mouse IgG1
Immunogen:	Recombinant Protein
Purification:	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Description:	Featured Anti-Histone H3 Mouse Monoclonal Antibody (2D10), specially designed for your immunoassay as internal control.
Further Information:	Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
NCBI EntrezGene:	8350/8351/8352/8353/8354/8355/8356/8357/8358/8968
Shipping Conditions:	Ship on cold packs
Storage:	Stable for one year at -20 C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Usage:	Research use only

Insight Biotechnology Ltd reserves the right to change pricing without notice

A01070-1.jpg&&Fig.1. Western blot analysis of 1) Hela, 2) Raw, 3) mouse brain tissue, 4) rat brain tissue, diluted at 1:5000.|||A01070-2.jpg&&Fig.2. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, Histone H3 Monoclonal Antibody (2D10) was diluted at 1:200 (4�C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98�C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.|||A01070-3.jpg&&Fig.3. Immunohistochemical analysis of paraffin-embedded mouse testis tissue. 1, Histone H3 Monoclonal Antibody (2D10) was diluted at 1:200 (4�C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98�C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.|||A01070-4.jpg&&Fig.4. Immunohistochemical analysis of paraffin-embedded rat testis tissue. 1, Histone H3 Monoclonal Antibody (2D10) was diluted at 1:200 (4�C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98�C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.|||A01070-5.jpg&&Fig.5. Immunofluorescence analysis of human liver cancer tissue. 1, Histone H3 Monoclonal Antibody (2D10) (red) was diluted at 1:200 (4�C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.|||A01070-6.jpg&&Fig.6. Immunofluorescence analysis of mouse liver tissue. 1, Histone H3 Monoclonal Antibody (2D10) (red) was diluted at 1:200 (4�C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.|||A01070-7.jpg&&Fig.7. Immunofluorescence analysis of rat liver tissue. 1, Histone H3 Monoclonal Antibody (2D10) (red) was diluted at 1:200 (4�C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.