A01060-1.jpg&&Fig.1. Western blot analysis of Hela, diluted at 1) 1:2000 2) 1:5000.|||A01060-2.jpg&&Fig.2. Immunohistochemical analysis of paraffin-embedded human uterus cancer tissue. 1, COX IV Monoclonal Antibody (14Y2) was diluted at 1:200 (4�C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98�C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.|||A01060-3.jpg&&Fig.3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue. 1, COX IV Monoclonal Antibody (14Y2) was diluted at 1:200 (4�C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98�C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.|||A01060-4.jpg&&Fig.4. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, COX IV Monoclonal Antibody (14Y2) was diluted at 1:200 (4�C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98�C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.|||A01060-5.jpg&&Fig.5. Immunofluorescence analysis of mouse kidney tissue. 1, COX IV Monoclonal Antibody (14Y2) (red) was diluted at 1:200 (4�C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.|||A01060-6.jpg&&Fig.6. Immunofluorescence analysis of rat kidney tissue. 1, COX IV Monoclonal Antibody (14Y2) (red) was diluted at 1:200 (4�C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
