A01040-1.jpg&&Fig.1. Western blot analysis of Hela (1), rat brain (2), NIH 3T3(3), 293T(4), diluted at 1:5000.|||A01040-2.jpg&&Fig.2. Immunofluorescence analysis of human lung cancer tissue. 1, PCNA Monoclonal Antibody (1D7) (red) was diluted at 1:200 (4�C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.|||A01040-3.jpg&&Fig.3. Immunofluorescence analysis of rat testis tissue. 1, PCNA Monoclonal Antibody (1D7) (red) was diluted at 1:200 (4�C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.|||A01040-4.jpg&&Fig.4. Immunohistochemical analysis of paraffin-embedded human uterus tissue. 1, PCNA Monoclonal Antibody (1D7) was diluted at 1:200 (4�C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98�C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.|||A01040-5.jpg&&Fig.5. Immunohistochemical analysis of paraffin-embedded mouse liver tissue. 1, PCNA Monoclonal Antibody (1D7) was diluted at 1:200 (4�C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98�C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.|||A01040-6.jpg&&Fig.6. Immunohistochemical analysis of paraffin-embedded rat testis tissue. 1, PCNA Monoclonal Antibod y(1D7) was diluted at 1:200 (4�C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98�C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
