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Cat Number:ABL1030-3
Size:200uL�5
Concentration:1 mg/ml
Price:£499.00
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Shipping:£12.00

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beta-Tubulin [3G6], Mouse Monoclonal Antibody

Partner: Abbkine Scientific

Applications:WB, IHC, IF, IP
Species Reactivity:Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Sheep, Insect, Yeast, Hamster
Dilutions:Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000), IHC-P (1:400), IF (1:400).
Isotype:Mouse IgG1
Immunogen:Synthetic Peptide
Purification:The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Description:Featured Anti-?-Tubulin Mouse Monoclonal Antibody (3G6), specially designed for your immunoassay as internal control or location staining.
Further Information:Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are alpha-tubulin and ?-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 kDa. Antibodies against ?-Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of ?-Tubulin may not be stable in certain cells, such as adipose tissue.
NCBI EntrezGene:10381
Shipping Conditions:Ship on cold packs
Storage:Stable for one year at -20 C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Usage:Research use only

 

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A01030-1.jpg&&Fig.1. Western blot analysis of A549(1), rat brain (2), mouse brain (3), chicken lung (4) and rabbit testis(5), sheep muscle(6), diluted at 1:10000.|||A01030-2.jpg&&Fig.2. Immunofluorescence analysis of human appendix tissue. 1, ?-Tubulin Monoclonal Antibody (3G6) (red) was diluted at 1:400 (4�C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.|||A01030-3.jpg&&Fig.3. Immunofluorescence analysis of mouse lung tissue. 1, ?-Tubulin Monoclonal Antibody (3G6) (red) was diluted at 1:400 (4�C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.|||A01030-4.jpg&&Fig.4. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, ?-Tubulin Monoclonal Antibody (3G6) was diluted at 1:400 (4�C, overnight). Negative control was used by secondary antibody only.|||A01030-5.jpg&&Fig.5. Immunohistochemical analysis of paraffin-embedded mouse testis tissue. 1, ?-Tubulin Monoclonal Antibody (3G6) was diluted at 1:400 (4�C, overnight). Negative control was used by secondary antibody only.|||A01030-6.jpg&&Fig.6. Immunohistochemical analysis of paraffin-embedded rat lung tissue. 1, ?-Tubulin Monoclonal Antibody (3G6) was diluted at 1:400 (4�C, overnight). Negative control was used by secondary antibody only.

A01030-1.jpg&&Fig.1. Western blot analysis of A549(1), rat brain (2), mouse brain (3), chicken lung (4) and rabbit testis(5), sheep muscle(6), diluted at 1:10000.|||A01030-2.jpg&&Fig.2. Immunofluorescence analysis of human appendix tissue. 1, ?-Tubulin Monoclonal Antibody (3G6) (red) was diluted at 1:400 (4�C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.|||A01030-3.jpg&&Fig.3. Immunofluorescence analysis of mouse lung tissue. 1, ?-Tubulin Monoclonal Antibody (3G6) (red) was diluted at 1:400 (4�C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.|||A01030-4.jpg&&Fig.4. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, ?-Tubulin Monoclonal Antibody (3G6) was diluted at 1:400 (4�C, overnight). Negative control was used by secondary antibody only.|||A01030-5.jpg&&Fig.5. Immunohistochemical analysis of paraffin-embedded mouse testis tissue. 1, ?-Tubulin Monoclonal Antibody (3G6) was diluted at 1:400 (4�C, overnight). Negative control was used by secondary antibody only.|||A01030-6.jpg&&Fig.6. Immunohistochemical analysis of paraffin-embedded rat lung tissue. 1, ?-Tubulin Monoclonal Antibody (3G6) was diluted at 1:400 (4�C, overnight). Negative control was used by secondary antibody only.