Ordering Details
Cat Number:	ABL1020-2
Size:	200uL
Concentration:	1 mg/ml
Price:	£109.00
Quantity:
Shipping:	£12.00

Document Links

MSDS

GAPDH [2B5] HRP, Mouse Monoclonal Antibody

Partner: Abbkine Scientific

Applications:	WB, IHC, IF, IP
Species Reactivity:	Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Pig, Sheep, Yeast, Hamster
Dilutions:	Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000), IHC-P (1:400), IF (1:400).
Isotype:	Mouse IgG1
Immunogen:	Synthetic Peptide
Purification:	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Description:	Featured Anti-GAPDH Mouse Monoclonal Antibody (2B5), specially designed for your immunoassay as internal control.
Further Information:	Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.
NCBI EntrezGene:	2597
Shipping Conditions:	Ship on cold packs
Storage:	Stable for one year at -20 C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Usage:	Research use only

Insight Biotechnology Ltd reserves the right to change pricing without notice

A01020-wb.jpg&&Fig.1. Western blot analysis of Hela (1), rat brain (2), rabbit muscle(3), sheep muscle(4), and mouse brain (5), diluted at 1:10000.|||A01020-2.jpg&&Fig.2. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4�C, overnight). Negative control was used by secondary antibody only.|||A01020-3.jpg&&Fig.3. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4�C, overnight). Negative control was used by secondary antibody only.|||A01020-4.jpg&&Fig.4. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4�C, overnight). Negative control was used by secondary antibody only.|||A01020-5.jpg&&Fig.5. Immunofluorescence analysis of human colon tissue. 1, GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4�C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.|||A01020-6.jpg&&Fig.6. Immunofluorescence analysis of mouse liver tissue. 1, GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4�C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.|||A01020-7.jpg&&Fig.7 Western blot analysis of Pig muscle tissue, GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25�C, 3h).|||A01020-8.jpg&&Fig.8 Western blot analysis of Hela, GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25�C, 3h).