Applications: | WB, IHC, IF, IP |
Species Reactivity: | Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Hamster, Insect |
Dilutions: | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:2000-1:5000). |
Isotype: | Mouse IgG1 |
Immunogen: | Synthetic Peptide |
Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
Conjugation: | HRP |
Description: | Featured HRP Conjugated Anti-?-Actin Mouse Monoclonal Antibody (1C7), specially designed for your Western blot as internal control. |
Further Information: | ?-Actin (gene name ACTB), a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. ?-actin is a major constituent of the contractile apparatus, which is usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western blotting. Its molecular weight is approximately 43 kDa. |
NCBI EntrezGene: | 60 |
Shipping Conditions: | Ship on cold packs |
Storage: | Stable for one year at -20 C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
Usage: | Research use only |