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Cat Number:ABL0813-50ul
Size:50uL
Concentration:1mg/mL
Price:£123.00
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Shipping:£12.00

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MSDS

 

BAFF/BLyS/TNFSF13B [H4-C7], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, FACS, ICC/IF
Species Reactivity:Human
Isotype:Mouse IgG3 kappa
Immunogen:Recombinant human BLyS (134-285aa) purified from E. coli
Purification:By protein-G affinity chromatography
Conjugation:Unconjugated
Description:B lymphocyte stimulator (BLyS), a member of the tumor necrosis factor (TNF) superfamily of cytokines, induces B-cell proliferation and immunoglobulin secretion and is a key regulator of peripheral B-cell populations in vivo.. BLyS is made by immune-cells called monocytes and macrophages. When monocytes and macrophages are activated, BLyS is released and binds to a receptor found only on B cells.
Further Information:The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.
Synonym:B lymphocyte stimulator, B-cell activating factor, BAFF, CD257, TALL1, THANK, ZTNF4, TNFSF13B, TNFSF20, BlyS, B lymphocyte stimulator, B lymphocyte stimulator
Ascension Number:Q9Y275
NCBI EntrezGene:NP_006564
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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Tissue lysates of Rat spleen (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BLyS (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Tissue lysates of Rat spleen (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BLyS (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

ICC/IF analysis of BLyS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BLyS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)

ICC/IF analysis of BLyS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BLyS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)