Western Blot: Shown are the results of WB on protein extracts from untransfected (A) and transfected (B) HEK293 cells using the Anti-CRISPR-Cas9 mAb.
Immunoprecipitation: HEK293T expressing N-terminally Flag-tagged S.pyogenes Cas9 were lysed 72 hours post transfection. Proteins were immunoprecipitated from 100 ?g of whole cell lysate for 1H at 4