Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with Flag-tagged Kirrel3-short, Kirrel3-long, untagged Kirrel2, Kirrel1 or Kv2.1 plasmid and probed with N321C/49 (left) and N52A/42 (right) TC supe.