MW: | Recombinant protein product has a calculated molecular mass of ∼35 kDa. Due to the abundant glycosylation, it migrates as approximately ∼60 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under denature conditions, the protein presented as one reduced ∼35 kDa band. See deglycosylation analysis image below. |