| Purity: | Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4�C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80�C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control. |
| Synonym: | C-C Motif Chemokine 2, C-C Motif Chemokine Ligand 2, Monocyte Chemotactic And Activating Factor, Monocyte Secretory Protein JE, Small Inducible Cytokine A2 (Monocyte Chemotactic Protein 1, Homologous To Mouse Sig-Je), Small Inducible Cytokine Subfamily A (Cys-Cys), Member 2, Monocyte Chemoattractant Protein-1, Chemokine (C-C Motif) Ligand 2, Monocyte Chemotactic Protein 1, MCP1, Monocyte Chemotactic Protein 1, Monocyte Chemoattractant Protein 1. |
