| Purification: | Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°,C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°,C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control. |
| Synonym: | Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha, Phosphoinositide-3-Kinase Regulatory Subunit 1 (Alpha), Phosphatidylinositol 3-Kinase 85 KDa Regulatory Subunit Alpha, PI3-Kinase Subunit P85-Alpha, GRB1, Phosphatidylinositol 3-Kinase-Associated P-85 Alpha, Phosphoinositide-3-Kinase Regulatory Subunit, PtdIns-3-Kinase Regulatory Subunit P85-Alpha, P85-ALPHA, IMD36, AGM7, P85. |
