Applications: | ELISA (recommended work dilution= 1: 32,000), Western Blotting (recommended work dilution= 1: 1,000-5,000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein PDGFbeta. The membrane blot was probed with anti- PDGFbeta primary antibody (0.2 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein PDGFbeta from E.coli cell (12kDa). |