| Applications: | ELISA (recommended work dilution= 1: 80,000), Western Blotting (recommended work dilution= 1:1000) , (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IGF-1, Lane 3, SP2/0 lysate. The blot was probed with anti-IGF-1primary antibody (1:1,000). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system. |
| Storage: | Store at 4 C if intended for use within one month, otherwise, store at -20 C to -80 C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20 C to -80 C. After reconstitution, it can be aliquoted and stored frozen at -20 C to -80 C in a manual defrost freezer for 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4 C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody. |
