| Applications: | ELISA (recommended work dilution= 15.6 ng/ml ), Western Blotting (recommended work dilution= 1:1,000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IL-6, Lane 3, 293T lysate, Lane 4, SP2/0 lysate. The membrane blot was probed with anti-IL-6 primary antibody (1 �g/ ml). Proteins were visualized using a Donkey antimouse secondary antibody conjugated to IRDye 800CW detection system. |
| Storage: | Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody. |
