Applications: | ELISA (recommended work dilution= 1: 20,000(at least detecting 50ng/ml)), Western Blotting (recommended work dilution= 1:1,000-5,000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. The membrane blot was probed with anti-Der P1 primary antibody (0.25µ,g/ml). Recombinant proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate recombinant immunogen Der P1 (15kDa). |