Applications: | FC, IHC(f), IHC (p) |
Species Reactivity: | Human |
Reactivity (Predicted): | The antibody recognizes human CD31. Other species not tested. |
Dilutions: | IHC: Use 1:50 dilution, FC: Use 1:50 dilution |
Isotype: | Mouse IgG1 |
Conjugation: | Unconjugated |
Description: | CD31 or PECAM-1 is a glycoprotein expressed on the cell surfaces of endothelial cells and platelets. It plays an important role in cell adhension. Anti-CD31 antibody is used to detect endothelial cells in tissue sections in applications such as measuring blood vessel density in tumors. |
Further Information: | The antibody was carefully tested for its ability to recognize human CD31. It works well with paraffin sections. For paraffin embedded sections, boil tissue sections in 1 mM EDTA, pH 8.0 for 10-20 min followed by cooling at RT for 20 min to retrieve antigens. The antibody also works in FACS sorting. |
Shipping Conditions: | Ship on cold packs |
Storage: | The antibody is stable at 4�C for >six month or can be stored in working aliquots at -20�C (add 50% glycerol) for more than a year. |
Usage: | Research use only |