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Cat Number:TT100314L
Size:5 x 1 ml
Price:£3767.00
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Shipping:£12.00

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Turbo-mRNA Transfection kit (5 X 1 mL)

Partner: OriGene Technologies

Description:Turbo-mRNA Transfection kit (5 vials of 1 mL Turbo-mRNA reagent and 5 vials of 1 mL mRNA Boost reagent)
Shipping Conditions:Ship at ambient
Usage:Research Use Only

 

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Efficient Genome Editing with Cas9 mRNA and verified gRNA. HEK293T/17, U2OS and NHDF cells were co-transfected with 0.5 µg of Cas9 encoding mRNA, and 25nM verified gRNA using Turbo-mRNA Transfection Kit (0.5 µl/well of 24-well plate of both mRNA Reagent and Boost, OriGene Catalog TT100314). A T7E1 mismatch detection assay was used to measure cleavage efficiency at 48 hours post-transfection.

Efficient Genome Editing with Cas9 mRNA and verified gRNA. HEK293T/17, U2OS and NHDF cells were co-transfected with 0.5 µg of Cas9 encoding mRNA, and 25nM verified gRNA using Turbo-mRNA Transfection Kit (0.5 µl/well of 24-well plate of both mRNA Reagent and Boost, OriGene Catalog TT100314). A T7E1 mismatch detection assay was used to measure cleavage efficiency at 48 hours post-transfection.