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Catalogue No Partner Description Species Application
188-10232-SAF RayBiotech, Inc. Mitochondria Marker Antibody [Sodium Azide Free], Mouse Monoclonal Antibody Human. Shows broad species reactivity including insects or bacteria. FACS: 0.5-1ug/million cells<br/>IF: 0.5-1ug/ml<br/>WB: 0.5-1ug/ml<br/>IHC (FFPE): 0.5-1ug/ml for 30 minutes at RT (1)<br/>Prediluted format: incubate for 30 min at RT (2)
188-10396-1 RayBiotech, Inc. Golgi Marker Antibody, Mouse Monoclonal Antibody Human. Does not react with Mouse and Rat. Other species not tested. Flow Cytometry: 0.5-1ug/million cells in 0.1ml<br/>Immunofluorescence: 0.5-1ug/ml<br/>Immunocytochemistry (Acetone or paraformaldehyde fixed): 0.5-1ug/ml for 30 min<br/>Western blot: 0.5-1.0ug/ml<br/>Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1)<br/>Prediluted format: incubate for 30 min at RT (2)
188-10396-SAF RayBiotech, Inc. Golgi Marker Antibody [Sodium Azide Free], Mouse Monoclonal Antibody Human. Does not react with Mouse and Rat. Other species not tested. Flow Cytometry: 0.5-1ug/million cells in 0.1ml<br/>Immunofluorescence: 0.5-1ug/ml<br/>Immunocytochemistry (Acetone or paraformaldehyde fixed): 0.5-1ug/ml for 30 min<br/>Western blot: 0.5-1.0ug/ml<br/>Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1)<br/>Prediluted format: incubate for 30 min at RT (2)
RB-01-0001B-50 RayBiotech, Inc. Mouse anti-bFGF/FGF basic/FGF2 [+Biotin], Mouse Monoclonal Antibody ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
188-10226-PE RayBiotech, Inc. Mouse anti-Human/Primates Nuclear Marker Antibody [PE Conjugated], Mouse Monoclonal Antibody Human and Primates. Does not react with mouse, rat and chicken. Other species not known. Flow Cytometry : 5ul/test/million cells or 5ul/test/100ul of whole blood<br/>Immunofluorescence: 1:50-1:100 for 30 minutes at RT
RB-08-0003B-50 RayBiotech, Inc. Visfatin [+Biotin], Rabbit Polyclonal Antibody ELISA (recommended work dilution= 1:10e6(at least detecting 10 �g/ml)), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Enzyme Immunoassay (EIA) (recommended work dilution= Sensitivity (100 �g/ml)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2.Reference control, Lane 3-4.Positive control, Hela and MD231 whole cell lysate, Lane 5.Negative control. The membrane blot was probed with antiVisfatin primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular Visfatin from human and mouse cells (52 kDa).
RB-08-0013B-50 RayBiotech, Inc. POMC [+Biotin], Rabbit Polyclonal Antibody ELISA (recommended work dilution= 1:10e5), Western Blotting (recommended work dilution= 1:1000-2000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2.Reference control, Lane 3. 3T3-L1 cell lysate, Lane 4. Hela cell lysate, Lane 5. NIH3T3 cell lysate. The membrane blot was probed with anti-POMC primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular POMC-associated protein from cells.