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Applications
(11)
(see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker
(4)
Lane 2.Reference control
(4)
(see image)Immunodetection Analysis: Lane 1.cell lysate MCF-7
(2)
(see image1) His Western Blot analysis of 293 cells transfected from with His-tagged vector (2) and untransfected control (1). (see image2) Immunofluorescence staining of His-tag fusion protein in 293 cells. A: transfected control
(2)
More...
(11)
(see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker (4)
Lane 2.Reference control (4)
(see image)Immunodetection Analysis: Lane 1.cell lysate MCF-7 (2)
(see image1) His Western Blot analysis of 293 cells transfected from with His-tagged vector (2) and untransfected control (1). (see image2) Immunofluorescence staining of His-tag fusion protein in 293 cells. A: transfected control (2)
B: transfected (2)
ELISA (recommended work dilution= 1:10e5) (2)
ELISA: By direct ELISA (2)
Hela and MD231 whole cell lysate (2)
Immunoprecipitation (recommended work dilution= 1:200) (2)
Immunostaining (recommended work dilution= 1:200) (2)
Lane 2-3.recombinant protein(10-1ug/lane) (2)
Lane 2. cell lysate A431 (2)
Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application. (2)
Lane 3-4.Positive control (2)
Lane 3. 3T3-L1 cell lysate (2)
Lane 3. cell lysate 293T (2)
Lane 4. Hela cell lysate (2)
Lane 4. positive control (2)
Lane 5. NIH3T3 cell lysate. The membrane blot was probed with anti-POMC primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular POMC-associated protein from cells. (2)
Conjugate
(16)
Biotin
(6)
(16)
Biotin (6)
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230-00782-10
RayBiotech, Inc.
Recombinant Human Cyclin-Dependent Kinase 1/CDK1
DS-01-0038
RayBiotech, Inc.
Native Bovine CALMODULIN