Western Blot using anti-Rhodopsin antibody Rho 1D4 (TA385917) Rat eye lysate (35µg protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of Rho 1D4 (TA385917) at 0.0001 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for monomeric rat ( Rattus norvegicus ) Rhodopsin is 39.0kDa, though this protein in known to form dimers and higher order multimers (c.f. Mansoor et al., PMID: 16492772, PMID: 10798675). TA385917 successfully detected monomeric, dimeric and multimeric Rhodopsin in rat eye lysate.
