HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CSF1R with C-terminal MYC tag probed with TA311324 (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1:1000) in Lane C. Mock-transfected HEK293 probed with TA311324 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.