| Applications: | ELISPOT, WB |
| Species Reactivity: | Rhesus, Cynomolgus, Chimpanzee, African Green Monkey, Mangabey, Pigtail |
| Dilutions: | Immunofluorescence:<br/>ELISA:<br/>Purified antibody<br/>FITC and BIOT conjugates<br/>R-PE conjugate<br/>APC conjugate<br/>SPRD conjugate<br/>CY5 conjugate<br/>AP conjugate<br/>HRP conjugate<br/>BIOT conjugate<br/><= 1 ug/10<sup>6</sup> cells<br/><= 1 ug/10<sup>6</sup> cells<br/><= 0.1 ug/10<sup>6</sup> cells<br/><= 0.1 ug/10<sup>6</sup> cells<br/><= 0.1 ug/10<sup>6</sup> cells<br/><= 0.3 ug/10<sup>6</sup> cells<br/>1:2,000-1:8,000<br/>1:4,000-1:8,000<br/>1:10,000-1:20,000 |
| Conjugation: | AP |
| Shipping Conditions: | Ship on cold packs |
| Storage: | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C<br/>- The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C<br/>- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C<br/>- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C<br/>- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C<br/>- The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!<br/> - The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!<br/> - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C<br/>- The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C<br/>- With the exception of the low endotoxin, azide- free product, the vials should be stored at 2- 8 C<br/>Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquotand freeze the low endotoxin, azide- free product at - 20 C immediately upon receipt. Each reagent isstable for the period shown on the bottle label if stored as directed. |
| Usage: | Research Use Only |
