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RB-05-0008B-50
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RayBiotech, Inc.
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Human anti-Escherichia Coli O157:H7 with anti-Myc tag [+Biotin]
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It specifically detects protein derived from testing bacterial sample at various concentrations. See image Below.
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ELISA (recommended work dilution= 1: 1,000-2,000), Western Blotting (recommended work dilution= 1~5 �g/ml), Dot Blotting(recommended work dilution= 1~5 �g/ml), (see image)The Purity and binding activity of recombinant antibody, anti-O157:H7 by Dot-blotting assay. A. O157 scFv binding activity. Directly spotting O157 antigen into membrane in Row I and II with two dilution (100 and 10 ng of each spot 2-3 ) plus positive system control in spotting blot 1 and 5 as well as BSA control in Spot 4, respectively, then probe the membrane by using O157:H7-specific recombinant antibody after blocking and following anti-Myc-tag and HRP-conjugated detection antibody. B. Checking purity of purified O157 scFv. Directly probed blocking blot with anti-Myctag and following HRP-conjugated detection antibodies.. Anti-Myc Tag Antibody Applications: Immunoassay (ELISA, Western blotting)(recommended work dilution= 1:2,000-1:5,000)
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RB-01-0001B-50
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RayBiotech, Inc.
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Mouse anti-bFGF/FGF basic/FGF2 [+Biotin], Mouse Monoclonal Antibody
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ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
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130-10008B-50
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RayBiotech, Inc.
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ESR1 [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 32,000(at least detecting 32 �g/ml)), Western Blotting (recommended work dilution= 1: 1,000-5,000(at least)), (see image)Immunodetection Analysis: Lane 1.cell lysate MCF-7, Lane 2. cell lysate A431, Lane 3. cell lysate 293T, Lane 4. positive control, ER protein. The membrane blot was probed with anti-ER primary antibody (0.2 �g/ml). The binding bands were visualized using AP-conjugated goat anti-rabbit secondary antibody and then adding NBT/BCIP substrate. Arrows indicate ER in tested cell lysates (about 40 kDa).
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RB-08-0003B-50
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RayBiotech, Inc.
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Visfatin [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1:10e6(at least detecting 10 �g/ml)), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Enzyme Immunoassay (EIA) (recommended work dilution= Sensitivity (100 �g/ml)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2.Reference control, Lane 3-4.Positive control, Hela and MD231 whole cell lysate, Lane 5.Negative control. The membrane blot was probed with antiVisfatin primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular Visfatin from human and mouse cells (52 kDa).
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RB-08-0013B-50
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RayBiotech, Inc.
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POMC [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1:10e5), Western Blotting (recommended work dilution= 1:1000-2000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2.Reference control, Lane 3. 3T3-L1 cell lysate, Lane 4. Hela cell lysate, Lane 5. NIH3T3 cell lysate. The membrane blot was probed with anti-POMC primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular POMC-associated protein from cells.
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RB-10-0002B-50
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RayBiotech, Inc.
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His Tag [+Biotin], Rabbit Polyclonal Antibody
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His-tag Polyclonal Antibody. Recognizes His- tagged recombinant proteins or His-tagged proteins over-expressed in cells.
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Immunostaining (recommended work dilution= 1:200), Western Blotting (recommended work dilution= 1:1000), Immunoprecipitation (recommended work dilution= 1:200), (see image1) His Western Blot analysis of 293 cells transfected from with His-tagged vector (2) and untransfected control (1). (see image2) Immunofluorescence staining of His-tag fusion protein in 293 cells. A: transfected control, B: transfected
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BNCB0315-100
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Biotium
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Heparan Sulphate Proteoglycan [A7L6] Biotin, Rat Monoclonal Antibody
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IHC(p)
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BNCB0315-500
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Biotium
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Heparan Sulphate Proteoglycan [A7L6] Biotin, Rat Monoclonal Antibody
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IHC(p)
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BNCB0452-100
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Biotium
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Vimentin [VM452] Biotin, Mouse Monoclonal Antibody
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FC, IHC(p), WB
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BNCB0452-500
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Biotium
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Vimentin [VM452] Biotin, Mouse Monoclonal Antibody
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FC, IHC(p), WB
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BNCB0596-100
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Biotium
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c-Myc [9E10.] Biotin, Mouse Monoclonal Antibody
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IHC(p)
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BNCB0596-500
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Biotium
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c-Myc [9E10.] Biotin, Mouse Monoclonal Antibody
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IHC(p)
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BNCB0672-100
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Biotium
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Cdc20 [AR12] Biotin, Mouse Monoclonal Antibody
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IHC(p)
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BNCB0672-500
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Biotium
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Cdc20 [AR12] Biotin, Mouse Monoclonal Antibody
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IHC(p)
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BNCB0673-100
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Biotium
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Cyclin A2 [E67] Biotin, Mouse Monoclonal Antibody
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IHC(p), WB
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BNCB0673-500
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Biotium
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Cyclin A2 [E67] Biotin, Mouse Monoclonal Antibody
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IHC(p), WB
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BNCB0811-100
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Biotium
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ZAP70 [2F3.2] Biotin, Mouse Monoclonal Antibody
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FC, IF, IHC(p)
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BNCB0811-500
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Biotium
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ZAP70 [2F3.2] Biotin, Mouse Monoclonal Antibody
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FC, IF, IHC(p)
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BNCB0909-100
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Biotium
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c-Myc [MYC909] Biotin, Mouse Monoclonal Antibody
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IHC(p)
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BNCB0909-500
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Biotium
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c-Myc [MYC909] Biotin, Mouse Monoclonal Antibody
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IHC(p)
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BNCB1031-100
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Biotium
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ALK [Anaplastic Lymphoma Kinase]/CD246 [ALK/1031] Biotin, Mouse Monoclonal Antibody
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WB
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BNCB1031-500
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Biotium
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ALK [Anaplastic Lymphoma Kinase]/CD246 [ALK/1031] Biotin, Mouse Monoclonal Antibody
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WB
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BNCB1032-100
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Biotium
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ALK [Anaplastic Lymphoma Kinase]/CD246 [ALK/1032] Biotin, Mouse Monoclonal Antibody
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WB
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BNCB1032-500
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Biotium
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ALK [Anaplastic Lymphoma Kinase]/CD246 [ALK/1032] Biotin, Mouse Monoclonal Antibody
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WB
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BNCB1075-100
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Biotium
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S100A9 [S100A9/1075] Biotin, Mouse Monoclonal Antibody
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IHC(p)
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