|
BNCB0669-100
|
Biotium
|
p27/KIP1 [SX53G8] Biotin, Mouse Monoclonal Antibody
|
|
IHC(p), FC, IF, IP, WB
|
|
|
BNCB0669-500
|
Biotium
|
p27/KIP1 [SX53G8] Biotin, Mouse Monoclonal Antibody
|
|
IHC(p), FC, IF, IP, WB
|
|
|
BNCB0811-100
|
Biotium
|
ZAP70 [2F3.2] Biotin, Mouse Monoclonal Antibody
|
|
FC, IF, IHC(p)
|
|
|
BNCB0811-500
|
Biotium
|
ZAP70 [2F3.2] Biotin, Mouse Monoclonal Antibody
|
|
FC, IF, IHC(p)
|
|
|
130-10096B-50
|
RayBiotech, Inc.
|
Mouse anti-MRSA [+Biotin], Mouse Monoclonal Antibody
|
|
ELISA (recommended work dilution= 1: 80,000), Western Blotting (recommended work dilution= 1:1000) , (see image) Western Blot: The membrane blot was probed with purified mouse anti-MRSA primary antibody (1:1,000), then with Anti-Mouse IgG secondary antibody conjugated to HRP (1:10,000). The detected protein was clearly visualized by chemiluminescence detection system. Lane 1 and 4. Lysates from two selected MRSA isolated strains, Lane 2-3. Lysates from two selected MSSA strains.
|
|
|
RB-01-0001B-50
|
RayBiotech, Inc.
|
Mouse anti-bFGF/FGF basic/FGF2 [+Biotin], Mouse Monoclonal Antibody
|
|
ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
|
|
|
RB-IP-10-101B-50
|
RayBiotech, Inc.
|
Mouse anti-HA-Tag [+Biotin], Mouse Monoclonal Antibody
|
HA- Tag antibody detects fusion recombinant proteins derived from a variety of sources at various concentrations. No Cross reactivity with cell lysates was not found in ELISA and Western Blot.
|
ELISA (recommended work dilution= 1:1000-5000), Western Blotting( recommended work dilution= 1-2 �g/ml)
|
|
|
130-10008B-50
|
RayBiotech, Inc.
|
ESR1 [+Biotin], Rabbit Polyclonal Antibody
|
|
ELISA (recommended work dilution= 1: 32,000(at least detecting 32 �g/ml)), Western Blotting (recommended work dilution= 1: 1,000-5,000(at least)), (see image)Immunodetection Analysis: Lane 1.cell lysate MCF-7, Lane 2. cell lysate A431, Lane 3. cell lysate 293T, Lane 4. positive control, ER protein. The membrane blot was probed with anti-ER primary antibody (0.2 �g/ml). The binding bands were visualized using AP-conjugated goat anti-rabbit secondary antibody and then adding NBT/BCIP substrate. Arrows indicate ER in tested cell lysates (about 40 kDa).
|
|
|
130-10010B-50
|
RayBiotech, Inc.
|
PAI-1 [+Biotin], Rabbit Polyclonal Antibody
|
Rabbit Anti-Human PAI-1 antibody detects a target derived from a human recombinant PAI-1 protein aswell as selected cell lysates. Cross reactivity with mouse and rat PAI-1 was not determined.
|
ELISA (recommended work dilution= 1: 20,000)
|
|
|
130-10013B-50
|
RayBiotech, Inc.
|
CT1 [+Biotin], Rabbit Polyclonal Antibody
|
Rabbit anti-Human Cardiotrophin-1 antibody detects specifically target derived from humanrecombinant protein as well as HepG2 cell lysates. Cross reactivity with mouse and rat CT-1 was nottested yet.
|
ELISA (recommended work dilution= 1:800,000(at least detecting 12.5 �g/ml), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein CT-1. Lane 2. HepG2 cell lysate. The membrane blot wasprobed with anti-CT-1 primary antibody(0.25 �g/ml).Proteins were visualized using a goat anti-rabbitsecondary antibody conjugated to HRP andchemiluminescence detection system. Arrows indicatecellular CT-1 from human and mouse cells (24 kDa).
|
|
|
RB-08-0031B-50
|
RayBiotech, Inc.
|
Apelin receptor (Middle) [+Biotin], Rabbit Polyclonal Antibody
|
Rabbit polyclonal antibody to Apelin Receptor can detect cellular protein derived from cell lysates (3T3-L1, NIH-3T3) at various concentrations. Cross reactivity with human and mouse might be happened duo to their sequence similarity .
|
ELISA (recommended work dilution= 1- 0.1 �g/ml), Western Blotting (recommended work dilution= 1:1000-2000 (at least))
|
|
|
BA1006-1ml
|
Boster Biological Technology
|
Rabbit anti-Goat IgG [H+L], Biotin
|
Goat
|
ELISA,IHC
|
|
