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RB-01-0001B-50
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RayBiotech, Inc.
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Mouse anti-bFGF/FGF basic/FGF2 [+Biotin], Mouse Monoclonal Antibody
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ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
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RB-10-0002B-50
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RayBiotech, Inc.
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His Tag [+Biotin], Rabbit Polyclonal Antibody
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His-tag Polyclonal Antibody. Recognizes His- tagged recombinant proteins or His-tagged proteins over-expressed in cells.
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Immunostaining (recommended work dilution= 1:200), Western Blotting (recommended work dilution= 1:1000), Immunoprecipitation (recommended work dilution= 1:200), (see image1) His Western Blot analysis of 293 cells transfected from with His-tagged vector (2) and untransfected control (1). (see image2) Immunofluorescence staining of His-tag fusion protein in 293 cells. A: transfected control, B: transfected
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BNCB0315-100
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Biotium
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Heparan Sulphate Proteoglycan [A7L6] Biotin, Rat Monoclonal Antibody
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IHC(p)
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BNCB0315-500
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Biotium
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Heparan Sulphate Proteoglycan [A7L6] Biotin, Rat Monoclonal Antibody
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IHC(p)
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BNCB0452-100
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Biotium
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Vimentin [VM452] Biotin, Mouse Monoclonal Antibody
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FC, IHC(p), WB
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BNCB0452-500
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Biotium
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Vimentin [VM452] Biotin, Mouse Monoclonal Antibody
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FC, IHC(p), WB
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BNCB0596-100
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Biotium
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c-Myc [9E10.] Biotin, Mouse Monoclonal Antibody
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IHC(p)
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BNCB0596-500
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Biotium
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c-Myc [9E10.] Biotin, Mouse Monoclonal Antibody
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IHC(p)
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BNCB0672-100
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Biotium
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Cdc20 [AR12] Biotin, Mouse Monoclonal Antibody
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IHC(p)
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BNCB0672-500
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Biotium
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Cdc20 [AR12] Biotin, Mouse Monoclonal Antibody
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IHC(p)
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BNCB0673-100
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Biotium
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Cyclin A2 [E67] Biotin, Mouse Monoclonal Antibody
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IHC(p), WB
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BNCB0673-500
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Biotium
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Cyclin A2 [E67] Biotin, Mouse Monoclonal Antibody
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IHC(p), WB
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BNCB0811-100
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Biotium
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ZAP70 [2F3.2] Biotin, Mouse Monoclonal Antibody
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FC, IF, IHC(p)
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BNCB0811-500
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Biotium
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ZAP70 [2F3.2] Biotin, Mouse Monoclonal Antibody
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FC, IF, IHC(p)
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BNCB0909-100
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Biotium
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c-Myc [MYC909] Biotin, Mouse Monoclonal Antibody
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IHC(p)
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BNCB0909-500
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Biotium
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c-Myc [MYC909] Biotin, Mouse Monoclonal Antibody
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IHC(p)
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BNCB1031-100
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Biotium
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ALK [Anaplastic Lymphoma Kinase]/CD246 [ALK/1031] Biotin, Mouse Monoclonal Antibody
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WB
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BNCB1031-500
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Biotium
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ALK [Anaplastic Lymphoma Kinase]/CD246 [ALK/1031] Biotin, Mouse Monoclonal Antibody
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WB
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BNCB1032-100
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Biotium
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ALK [Anaplastic Lymphoma Kinase]/CD246 [ALK/1032] Biotin, Mouse Monoclonal Antibody
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WB
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BNCB1032-500
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Biotium
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ALK [Anaplastic Lymphoma Kinase]/CD246 [ALK/1032] Biotin, Mouse Monoclonal Antibody
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WB
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BNCB1075-100
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Biotium
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S100A9 [S100A9/1075] Biotin, Mouse Monoclonal Antibody
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IHC(p)
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BNCB1075-500
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Biotium
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S100A9 [S100A9/1075] Biotin, Mouse Monoclonal Antibody
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IHC(p)
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BNCB1093-100
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Biotium
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S100A9 [47-8D3] Biotin, Mouse Monoclonal Antibody
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WB
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BNCB1093-500
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Biotium
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S100A9 [47-8D3] Biotin, Mouse Monoclonal Antibody
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WB
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BNCB1102-100
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Biotium
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Cdc20 [CDC20/1102] Biotin, Mouse Monoclonal Antibody
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IHC(p)
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