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GTX60994-02
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GeneTex International Corporation
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ENaC Gamma [5c2] (Biotin), Mouse Monoclonal Antibody
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Human
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ELISA, IHC-P
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RB-05-0008B-50
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RayBiotech, Inc.
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Human anti-Escherichia Coli O157:H7 with anti-Myc tag [+Biotin]
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It specifically detects protein derived from testing bacterial sample at various concentrations. See image Below.
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ELISA (recommended work dilution= 1: 1,000-2,000), Western Blotting (recommended work dilution= 1~5 �g/ml), Dot Blotting(recommended work dilution= 1~5 �g/ml), (see image)The Purity and binding activity of recombinant antibody, anti-O157:H7 by Dot-blotting assay. A. O157 scFv binding activity. Directly spotting O157 antigen into membrane in Row I and II with two dilution (100 and 10 ng of each spot 2-3 ) plus positive system control in spotting blot 1 and 5 as well as BSA control in Spot 4, respectively, then probe the membrane by using O157:H7-specific recombinant antibody after blocking and following anti-Myc-tag and HRP-conjugated detection antibody. B. Checking purity of purified O157 scFv. Directly probed blocking blot with anti-Myctag and following HRP-conjugated detection antibodies.. Anti-Myc Tag Antibody Applications: Immunoassay (ELISA, Western blotting)(recommended work dilution= 1:2,000-1:5,000)
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RB-01-0001B-50
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RayBiotech, Inc.
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Mouse anti-bFGF/FGF basic/FGF2 [+Biotin], Mouse Monoclonal Antibody
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ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
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RB-08-0003B-50
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RayBiotech, Inc.
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Visfatin [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1:10e6(at least detecting 10 �g/ml)), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Enzyme Immunoassay (EIA) (recommended work dilution= Sensitivity (100 �g/ml)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2.Reference control, Lane 3-4.Positive control, Hela and MD231 whole cell lysate, Lane 5.Negative control. The membrane blot was probed with antiVisfatin primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular Visfatin from human and mouse cells (52 kDa).
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BNCB0596-100
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Biotium
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c-Myc [9E10.] Biotin, Mouse Monoclonal Antibody
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IHC(p)
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BNCB0596-500
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Biotium
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c-Myc [9E10.] Biotin, Mouse Monoclonal Antibody
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IHC(p)
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BNCB0673-100
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Biotium
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Cyclin A2 [E67] Biotin, Mouse Monoclonal Antibody
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IHC(p), WB
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BNCB0673-500
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Biotium
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Cyclin A2 [E67] Biotin, Mouse Monoclonal Antibody
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IHC(p), WB
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BNCB1603-100
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Biotium
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CD1c [CD1C/1603] Biotin, Mouse Monoclonal Antibody
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BNCB1603-500
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Biotium
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CD1c [CD1C/1603] Biotin, Mouse Monoclonal Antibody
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BNCB1701-100
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Biotium
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Rb1 [Tumor Suppressor Protein] [1F8] Biotin, Mouse Monoclonal Antibody
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IHC(p)
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BNCB1701-500
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Biotium
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Rb1 [Tumor Suppressor Protein] [1F8] Biotin, Mouse Monoclonal Antibody
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IHC(p)
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BNCB1754-100
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Biotium
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Rb1 [Tumor Suppressor Protein] [RB1/1754] Biotin, Mouse Monoclonal Antibody
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BNCB1754-500
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Biotium
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Rb1 [Tumor Suppressor Protein] [RB1/1754] Biotin, Mouse Monoclonal Antibody
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BNCB1926-100
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Biotium
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SOX9/SRY-box 9 [PCRP-SOX9-1E5] Biotin, Mouse Monoclonal Antibody
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BNCB1926-500
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Biotium
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SOX9/SRY-box 9 [PCRP-SOX9-1E5] Biotin, Mouse Monoclonal Antibody
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BNCB1927-100
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Biotium
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SOX9/SRY-box 9 [PCRP-SOX9-1A2] Biotin, Mouse Monoclonal Antibody
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BNCB1927-500
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Biotium
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SOX9/SRY-box 9 [PCRP-SOX9-1A2] Biotin, Mouse Monoclonal Antibody
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BNCB2051-100
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Biotium
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TLE1 [Synovial Sarcoma Marker] [TLE1/2051] Biotin, Mouse Monoclonal Antibody
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IHC(p), WB
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BNCB2051-500
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Biotium
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TLE1 [Synovial Sarcoma Marker] [TLE1/2051] Biotin, Mouse Monoclonal Antibody
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IHC(p), WB
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BNCB2062-100
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Biotium
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TLE1 [Synovial Sarcoma Marker] [TLE1/2062] Biotin, Mouse Monoclonal Antibody
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IHC(p), WB
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BNCB2062-500
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Biotium
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TLE1 [Synovial Sarcoma Marker] [TLE1/2062] Biotin, Mouse Monoclonal Antibody
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IHC(p), WB
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BNCB2085-100
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Biotium
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TLE1 [Synovial Sarcoma Marker] [TLE1/2085] Biotin, Mouse Monoclonal Antibody
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IHC(p), WB
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BNCB2085-500
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Biotium
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TLE1 [Synovial Sarcoma Marker] [TLE1/2085] Biotin, Mouse Monoclonal Antibody
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IHC(p), WB
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BNCB2313-100
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Biotium
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Rb1 [Tumor Suppressor Protein] [RB1/2313R] Biotin, Rabbit Monoclonal Antibody
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IHC(p)
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