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Catalogue No	Partner	Description	Species	Application
188-10069-1	RayBiotech, Inc.	Human/Cynomolgus/Rhesus Monkey GM-CSF, Rat Monoclonal Antibody	Human, Cynomolgus and Rhesus Monkey. Other species not known.	Functional Activity (Neutralization): neutralizes GM-CSF bioactivity<br/>FACS: 0.5-1ug/million cells<br/>IF: 0.5-1ug/ml<br/>WB: 0.5-1ug/ml<br/>IP: 0.5-1�g/500ug protein lysate<br/>IHC (Frozen): 0.5-1ug/ml for 30 minutes at RT
188-10069-SAF	RayBiotech, Inc.	Human/Cynomolgus/Rhesus Monkey GM-CSF [Sodium Azide Free], Rat Monoclonal Antibody	Human, Cynomolgus and Rhesus Monkey. Other species not known.	Functional Activity (Neutralization): neutralizes GM-CSF bioactivity<br/>FACS: 0.5-1ug/million cells<br/>IF: 0.5-1ug/ml<br/>WB: 0.5-1ug/ml<br/>IP: 0.5-1�g/500ug protein lysate<br/>IHC (Frozen): 0.5-1ug/ml for 30 minutes at RT
130-10000-100	RayBiotech, Inc.	Rabbit anti-Human Androgen Receptor		ELISA (recommended work dilution= 1: 40,000(at least detecting 25ng/ml)), Western Blotting (recommended work dilution= 1:1,000-5,000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.Androgen receptor protein ?g, Lane 2.MCF7 cell lysate , Lane 3.BSA 2?g. The membrane blot was probed with anti-Androgen receptor primary antibody(0.25?g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular androgen receptor from human cells (70-80 kDa).
130-10008-100	RayBiotech, Inc.	Rabbit anti-Human ESR1		ELISA (recommended work dilution= 1: 32,000(at least detecting 32ng/ml)), Western Blotting (recommended work dilution= 1: 1,000-5,000(at least)), (see image)Immunodetection Analysis: Lane 1.cell lysate MCF-7, Lane 2. cell lysate A431, Lane 3. cell lysate 293T, Lane 4. positive control, ER protein. The membrane blot was probed with anti-ER primary antibody (0.2?g/ml). The binding bands were visualized using AP-conjugated goat anti-rabbit secondary antibody and then adding NBT/BCIP substrate. Arrows indicate ER in tested cell lysates (about 40 kDa).
130-10009-100	RayBiotech, Inc.	Rabbit anti-Human MIF		ELISA (recommended work dilution= 1: 40,000(at least detecting 25ng/ml)), Western Blotting (recommended work dilution= 1:1,000-5,000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein MIF, Lane 2.Jurkat cell lysate. The membrane blot was probed with anti-MIF primary antibody(0.25?g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular MIF from human and mouse cells (20 kDa).
130-10010-100	RayBiotech, Inc.	Rabbit anti-Human PAI-1	Rabbit Anti-Human PAI-1 antibody detects a target derived from a human recombinant PAI-1 protein aswell as selected cell lysates. Cross reactivity with mouse and rat PAI-1 was not determined.	ELISA (recommended work dilution= 1: 20,000)
130-10013-100	RayBiotech, Inc.	Rabbit anti-Human CT1	Rabbit anti-Human Cardiotrophin-1 antibody detects specifically target derived from humanrecombinant protein as well as HepG2 cell lysates. Cross reactivity with mouse and rat CT-1 was nottested yet.	ELISA (recommended work dilution= 1:800,000(at least detecting 12.5 ng/ml), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein CT-1. Lane 2. HepG2 cell lysate. The membrane blot wasprobed with anti-CT-1 primary antibody(0.25?g/ml).Proteins were visualized using a goat anti-rabbitsecondary antibody conjugated to HRP andchemiluminescence detection system. Arrows indicatecellular CT-1 from human and mouse cells (24 kDa).
130-10015-100	RayBiotech, Inc.	Rabbit anti-Human Pro-BNP		ELISA (recommended work dilution= 1: 20,000(at least detecting 50ng/ml)), Western Blotting (recommended work dilution= 1:1,000-5,000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein proBNP, lane 2. T98G cell lysate. The membrane blot was probed with anti-proBNP primary antibody (0.25?g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular proBNP from human and mouse cells (35 kDa).
130-10062-100	RayBiotech, Inc.	Rabbit anti-Human CKB		ELISA (recommended work dilution= 1: 40,000), Western Blotting (recommended work dilution= 1: 1,000), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1, Hela cell lysate, Lane 2, 293T cell lysate l, Lane 3, recombinant protein CKB. The membrane blot was probed with anti-CKB primary antibody. Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate cellular CKB from human and mouse cells (43 kDa).
130-10063-100	RayBiotech, Inc.	Mouse anti-Human IGF-1		ELISA (recommended work dilution= 6.25 ng/ml ), Western Blotting (recommended work dilution= 1:2000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IGF-1, Lane 3, 3T3-L-1 lysate. The membrane blot was probed with anti-IGF-1 primary antibody (1 �g/ ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection system.
188-10184-1	RayBiotech, Inc.	Rabbit anti-Human/Mouse/Rat/Horse/Cow/Dog TIMP3 Antibody	Human, Mouse, Rat, Horse, Cow and Dog. Other species not known.	FACS: use antibody at 0.5-1ug/million cells<br/>IF: 1-2ug/ml<br/>WB: 0.5-1ug/ml<br/>IP: 1-2ug/500ug protein lysate<br/>Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT (1)<br/>Prediluted format: incubate for 30 min at RT (2)
188-10184-SAF	RayBiotech, Inc.	Rabbit anti-Human/Mouse/Rat/Horse/Cow/Dog TIMP3 Antibody [Sodium Azide Free]	Human, Mouse, Rat, Horse, Cow and Dog. Other species not known.	FACS: use antibody at 0.5-1ug/million cells<br/>IF: 1-2ug/ml<br/>WB: 0.5-1ug/ml<br/>IP: 1-2ug/500ug protein lysate<br/>Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT (1)<br/>Prediluted format: incubate for 30 min at RT (2)
MD-02-0032	RayBiotech, Inc.	Mouse anti-Heat Shock Protein-20		Suitable for in ELISA, Western blot and immunofluorescent cell culture staining.
MD-05-0135	RayBiotech, Inc.	Mouse anti-E. coli Shigatoxin (STX) 1 & 2		Suitable for use in ELISA (capture antibody at 20&micro,g/ml), Western blot (1&micro,g/ml) and Toxin Neutralization. Neutralizes Vero Cell toxicity of E. coli O157:H7 extracts when used at a 5 &micro,g/ml concentration. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
MD-17-0007	RayBiotech, Inc.	Rabbit anti-Brain Natriuretic Peptide (BNP) / NPPB		The antiserum has been characterized by RIA, ELISA and cell staining techniques. Suitable for immunohistochemical techniques. Has been found to stain 3% buffered paraformaldehyde fixed rat brain sections using indirect immunofluorescent procedure at a dilution of 1:1000.
MD-19-0128	RayBiotech, Inc.	Rabbit anti-Nicotinic, alpha-5 Receptor / CHRNA5		This antisera has been characterized by Western blotting, ELISA, and cell staining techniques. Suitable for immunocytochemical and western immunoblotting detection of the receptor.
RB-01-0001-100	RayBiotech, Inc.	Mouse anti-Human bFGF/FGF basic/FGF2		ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
RB-01-0006-100	RayBiotech, Inc.	Mouse anti-Human TNF alpha	Human TNFalpha monoclonal antibody detects protein derived from human, Jurkat cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.	Direct ELISA: antibody detection limit dilution 1:10,000-20,000, Recommended for use at 5-10 ng/ml., Western Blot: Approx 26 and 17 kDa band observed in Jurkat cell lysate. this antibody can be used at 1 - 2 ?g/mL with the appropriate secondary reagents to detect human TNF-alpha.,
RB-02-0004-100	RayBiotech, Inc.	Rabbit anti-Human EGFR/ErbB1 (pSer1045)		ELISA (recommended work dilution= 1: 5,000-10,000), Western Blotting (recommended work dilution= 1-5 �g/ml), (see image) RB-02-0002 (1-5 �g/ml) staining of Hela and A431 lysate. Lane 1 and 2, Hela lysate with and without stimulating, Land 3 and 4, A431 lysate with and without stimulating. Detected by western blot using chemiluminescence.
RB-08-0003-100	RayBiotech, Inc.	Rabbit anti-Human Visfatin		ELISA (recommended work dilution= 1:10e6(at least detecting 10ng/ml)), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Enzyme Immunoassay (EIA) (recommended work dilution= Sensitivity (100pg/ml)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2.Reference control, Lane 3-4.Positive control, Hela and MD231 whole cell lysate, Lane 5.Negative control. The membrane blot was probed with antiVisfatin primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular Visfatin from human and mouse cells (52 kDa).
RB-08-0004-100	RayBiotech, Inc.	Rabbit anti-Human Apelin ligand (N-terminus)	Human Apelin polyclonal antibody detects protein derived from human cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.	(under testing)Direct ELISA: antibody detection limit dilution will be 1:10,000-20,000. Western Blot: This antibody will be used at 1 - 2 �g/mL with the appropriate secondary reagents to detect human Apelin. Immunohistochemistry: under testing
RB-08-0007-100	RayBiotech, Inc.	Rabbit anti-Human Amylin	Human Amylin polyclonal antibody detects protein derived from human cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.	ELISA (recommended work dilution= 1: 5,000), Western Blotting (recommended work dilution= 1: 1,000)
RB-08-0013-100	RayBiotech, Inc.	Rabbit anti-Human POMC		ELISA (recommended work dilution= 1:10e5), Western Blotting (recommended work dilution= 1:1000-2000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2.Reference control, Lane 3. 3T3-L1 cell lysate, Lane 4. Hela cell lysate, Lane 5. NIH3T3 cell lysate. The membrane blot was probed with anti-POMC primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular POMC-associated protein from cells.
RB-08-0031-100	RayBiotech, Inc.	Rabbit anti-Human Apelin receptor(Middle)	Rabbit polyclonal antibody to Apelin Receptor can detect cellular protein derived from cell lysates (3T3-L1, NIH-3T3) at various concentrations. Cross reactivity with human and mouse might be happened duo to their sequence similarity .	ELISA (recommended work dilution= 1- 0.1?g/ml), Western Blotting (recommended work dilution= 1:1000-2000 (at least))
RB-10-0002-100	RayBiotech, Inc.	Rabbit anti-His Tag Antibody	His-tag Polyclonal Antibody. Recognizes His- tagged recombinant proteins or His-tagged proteins over-expressed in cells.	Immunostaining (recommended work dilution= 1:200), Western Blotting (recommended work dilution= 1:1000), Immunoprecipitation (recommended work dilution= 1:200), (see image1) His Western Blot analysis of 293 cells transfected from with His-tagged vector (2) and untransfected control (1). (see image2) Immunofluorescence staining of His-tag fusion protein in 293 cells. A: transfected control, B: transfected