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| Catalogue No |
Partner |
Description |
Species |
Application |
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RB-01-0001-100
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RayBiotech, Inc.
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Mouse anti-Human bFGF/FGF basic/FGF2
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ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
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RB-10-0001-100
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RayBiotech, Inc.
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Rabbit anti-E-tag
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RB-10-00001 recognizes over-expressed proteins containing E epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
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ELISA (recommended work dilution= 1:500-1000), Western Blotting (recommended work dilution= 1:250-500), Immunostaining (recommended work dilution= 1:50-100)
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RB-01-0001B-50
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RayBiotech, Inc.
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Mouse anti-bFGF/FGF basic/FGF2 [+Biotin], Mouse Monoclonal Antibody
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ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
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RB-10-0001B-50
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RayBiotech, Inc.
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E-tag [+Biotin], Rabbit Polyclonal Antibody
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RB-10-00001 recognizes over-expressed proteins containing E epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
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ELISA (recommended work dilution= 1:500-1000), Western Blotting (recommended work dilution= 1:250-500), Immunostaining (recommended work dilution= 1:50-100)
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