Applications: | IHC, IP, WB |
Species Reactivity: | Mouse |
Reactivity (Predicted): | The antibody does not cross-react with human podoplanin. Other species not tested. |
Dilutions: | IHC: Use 1:50 to 1:200 dilution, IP: Use 1:50 dilution, WB: Use 1:100 dilution or more |
Isotype: | Syrian Hamster IgG |
Purification: | Protein G purified IgG |
Conjugation: | Unconjugated |
Description: | Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. This hamster monoclonal antibody recognizes the mouse podoplanin on cell surfaces. |
Further Information: | The monoclonal antibody will detect podoplanin on the surface of mouse lymphatic endothelial cells and some epithelial cell types by immunostaining or immunohistochemistry. It works well with paraffin sections. The antibody will detect a 36-40 kDa band in Western blotting. It also works with immunoprecipitation. |
Shipping Conditions: | Ship on cold packs |
Storage: | The antibody can be reconstituted in 0.2 ml water and is stable at 4�C for six month or can be stored in working aliquots at -20�C (add 50% glycerol) for years. |
Usage: | Research use only |