To detect mIP-10 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with the Polyclonal Anti-Murine IP-10 PP1083P1/PP1083P2 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mIP-10.