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RB-01-0001B-50
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RayBiotech, Inc.
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Mouse anti-bFGF/FGF basic/FGF2 [+Biotin], Mouse Monoclonal Antibody
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ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
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RB-01-0006B-50
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RayBiotech, Inc.
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Mouse anti-TNF alpha [+Biotin], Mouse Monoclonal Antibody
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Human TNFalpha monoclonal antibody detects protein derived from human, Jurkat cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.
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Direct ELISA: antibody detection limit dilution 1:10,000-20,000, Recommended for use at 5-10 �g/ml., Western Blot: Approx 26 and 17 kDa band observed in Jurkat cell lysate. this antibody can be used at 1 - 2 �g/ml with the appropriate secondary reagents to detect human TNF-alpha.,
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RB-IP-10-101B-50
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RayBiotech, Inc.
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Mouse anti-HA-Tag [+Biotin], Mouse Monoclonal Antibody
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HA- Tag antibody detects fusion recombinant proteins derived from a variety of sources at various concentrations. No Cross reactivity with cell lysates was not found in ELISA and Western Blot.
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ELISA (recommended work dilution= 1:1000-5000), Western Blotting( recommended work dilution= 1-2 �g/ml)
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130-10000B-50
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RayBiotech, Inc.
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Androgen Receptor [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 40,000(at least detecting 25 �g/ml)), Western Blotting (recommended work dilution= 1:1,000-5,000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.Androgen receptor protein ?g, Lane 2.MCF7 cell lysate , Lane 3.BSA 2?g. The membrane blot was probed with anti-Androgen receptor primary antibody(0.25 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular androgen receptor from human cells (70-80 kDa).
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130-10008B-50
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RayBiotech, Inc.
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ESR1 [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 32,000(at least detecting 32 �g/ml)), Western Blotting (recommended work dilution= 1: 1,000-5,000(at least)), (see image)Immunodetection Analysis: Lane 1.cell lysate MCF-7, Lane 2. cell lysate A431, Lane 3. cell lysate 293T, Lane 4. positive control, ER protein. The membrane blot was probed with anti-ER primary antibody (0.2 �g/ml). The binding bands were visualized using AP-conjugated goat anti-rabbit secondary antibody and then adding NBT/BCIP substrate. Arrows indicate ER in tested cell lysates (about 40 kDa).
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130-10009B-50
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RayBiotech, Inc.
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MIF [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 40,000(at least detecting 25 �g/ml)), Western Blotting (recommended work dilution= 1:1,000-5,000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein MIF, Lane 2.Jurkat cell lysate. The membrane blot was probed with anti-MIF primary antibody(0.25 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular MIF from human and mouse cells (20 kDa).
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130-10010B-50
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RayBiotech, Inc.
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PAI-1 [+Biotin], Rabbit Polyclonal Antibody
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Rabbit Anti-Human PAI-1 antibody detects a target derived from a human recombinant PAI-1 protein aswell as selected cell lysates. Cross reactivity with mouse and rat PAI-1 was not determined.
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ELISA (recommended work dilution= 1: 20,000)
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130-10013B-50
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RayBiotech, Inc.
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CT1 [+Biotin], Rabbit Polyclonal Antibody
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Rabbit anti-Human Cardiotrophin-1 antibody detects specifically target derived from humanrecombinant protein as well as HepG2 cell lysates. Cross reactivity with mouse and rat CT-1 was nottested yet.
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ELISA (recommended work dilution= 1:800,000(at least detecting 12.5 �g/ml), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein CT-1. Lane 2. HepG2 cell lysate. The membrane blot wasprobed with anti-CT-1 primary antibody(0.25 �g/ml).Proteins were visualized using a goat anti-rabbitsecondary antibody conjugated to HRP andchemiluminescence detection system. Arrows indicatecellular CT-1 from human and mouse cells (24 kDa).
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130-10015B-50
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RayBiotech, Inc.
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Pro-BNP [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 20,000(at least detecting 50 �g/ml)), Western Blotting (recommended work dilution= 1:1,000-5,000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein proBNP, lane 2. T98G cell lysate. The membrane blot was probed with anti-proBNP primary antibody (0.25 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular proBNP from human and mouse cells (35 kDa).
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130-10062B-50
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RayBiotech, Inc.
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CKB [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 40,000), Western Blotting (recommended work dilution= 1: 1,000), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1, Hela cell lysate, Lane 2, 293T cell lysate l, Lane 3, recombinant protein CKB. The membrane blot was probed with anti-CKB primary antibody. Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate cellular CKB from human and mouse cells (43 kDa).
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130-10067B-50
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RayBiotech, Inc.
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GPBB [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 80,000), Western Blotting (recommended work dilution= 1:1000), Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein GPBB, Lane 2, HepG2 cell lysate. The membrane blot was probed with anti-GPBB primary antibody (1 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate cellular from Human cells (95 kDa).
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130-10068B-50
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RayBiotech, Inc.
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MB [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 80,000), Western Blotting (recommended work dilution= 1:1000) , Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein MB, Lane 2, mouse heart cell lysate, Lane 3, mouse muscle cell lysate. The membrane blot was probed with antiMB primary antibody (1 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate cellular from Mouse cells (17 kDa).
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130-10126B-50
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RayBiotech, Inc.
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Cyclin D1 (CCND1) [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 64,000), Western Blotting (recommended work dilution= 1:1,000-5,000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein CCND1.The membrane blot was probed with antiCCND1 primary antibody (0.2 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein CCND1 from E.coli cell (35kDa).
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130-10127B-50
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RayBiotech, Inc.
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matrix metalloproteinase-10 (MMP10) [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 16000), Western Blotting (recommended work dilution= 1: 1,000-5,000), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein MMP10. The membrane blot was probed with antiMMP10 primary antibody (0.2 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein MMP10 from E.coli cell (55kDa).
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130-10128B-50
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RayBiotech, Inc.
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Alkaline phosphatase (ALPP) [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 128,000) , Western Blotting (recommended work dilution= 1: 1,000-5,000) , Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein ALPP. The membrane blot was probed with antiALPP primary antibody (0.2 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein ALPP from E.coli cell (57kDa).
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130-10129B-50
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RayBiotech, Inc.
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chorionic gonadotropin (CGB) [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1:160,000), Western Blotting (recommended work dilution= 1: 1,000-5,000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein HCG-beta. The membrane blot was probed with antiHCG-beta primary antibody (0.2 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein HCG-beta from E.coli cell (18kDa).
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130-10130B-50
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RayBiotech, Inc.
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Cathepsin D (CTSD) [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1:320,000), Western Blotting (recommended work dilution= 1: 1,000-5,000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein Cathepsin D. The membrane blot was probed with anti- Cathepsin D primary antibody (0.2 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein Cathepsin D from E.coli cell (44kDa).
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130-10131B-50
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RayBiotech, Inc.
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Betacellulin [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 80,000) , Western Blotting (recommended work dilution= 1: 1,000-5,000) , Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein BTC-beta. The membrane blot was probed with anti BTC-beta primary antibody (0.2 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein BTC-beta from E.coli cell (20kDa).
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130-10138B-50
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RayBiotech, Inc.
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Heat shock protein 1 (HSPE1) [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1:32,000), Western Blotting (recommended work dilution= 1: 1,000-5,000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant proteinHSP10. The membrane blot was probed with anti-HSP10 primary antibody (0.2 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant proteinHSP10 from E.coli cell (12kDa).
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130-10139B-50
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RayBiotech, Inc.
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Platelet-derived growth factor subunit B (PDGFB) [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 32,000), Western Blotting (recommended work dilution= 1: 1,000-5,000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein PDGFbeta. The membrane blot was probed with anti- PDGFbeta primary antibody (0.2 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein PDGFbeta from E.coli cell (12kDa).
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130-10140B-50
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RayBiotech, Inc.
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Neurotrophin (NGFbeta) [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 128,000) , Western Blotting (recommended work dilution= 1: 1,000-5,000) , Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein beta-NGF. The membrane blot was probed with antiALPP primary antibody (0.2 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein beta-NGF from E.coli cell (14kDa).
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130-10142B-50
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RayBiotech, Inc.
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Platelet-derived growth factor subunit A (PDGFA) [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1:32,000), Western Blotting (recommended work dilution= 1: 1,000-5,000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein PDGFA. The membrane blot was probed with anti-PDGFA primary antibody (0.2 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein PDGFA from E.coli cell (14kDa).
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130-10144B-50
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RayBiotech, Inc.
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Metalloproteinase inhibitor 4 (TIMP4) [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 4,000), Western Blotting (recommended work dilution= 1: 1,000-5,000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein TIMP4.The membrane blot was probed with anti-TIMP4 primary antibody (0.2 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein TIMP4 from E.coli cell (26kDa).
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130-10145B-50
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RayBiotech, Inc.
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Adiponectin (ADIPOQ) [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 8,000), Western Blotting (recommended work dilution= 1:1000-5,000) , (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein ADIPOQ.The membrane blot was probed with anti-ADIPOQ primary antibody (0.2 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant proteinADIPOQ from E.coli cell (15kDa).
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130-10146B-50
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RayBiotech, Inc.
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Inhibin Alpha (INHa) [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1:8,000) , Western Blotting (recommended work dilution= 1: 1,000-5,000) , Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein INHalpha . The membrane blot was probed with antiALPP primary antibody (0.2 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein INHalpha from E.coli cell (12kDa).
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