Applications: | ELISA (recommended work dilution= 1: 128,000) , Western Blotting (recommended work dilution= 1: 1,000-5,000) , Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein ALPP. The membrane blot was probed with antiALPP primary antibody (0.2 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein ALPP from E.coli cell (57kDa). |