Applications: | ELISA (recommended work dilution= 1: 64,000), Western Blotting (recommended work dilution= 1:1,000-5,000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein CCND1.The membrane blot was probed with antiCCND1 primary antibody (0.2 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein CCND1 from E.coli cell (35kDa). |