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Catalogue No	Partner	Description	Species	Application
130-10008-100	RayBiotech, Inc.	Rabbit anti-Human ESR1		ELISA (recommended work dilution= 1: 32,000(at least detecting 32ng/ml)), Western Blotting (recommended work dilution= 1: 1,000-5,000(at least)), (see image)Immunodetection Analysis: Lane 1.cell lysate MCF-7, Lane 2. cell lysate A431, Lane 3. cell lysate 293T, Lane 4. positive control, ER protein. The membrane blot was probed with anti-ER primary antibody (0.2?g/ml). The binding bands were visualized using AP-conjugated goat anti-rabbit secondary antibody and then adding NBT/BCIP substrate. Arrows indicate ER in tested cell lysates (about 40 kDa).
130-10010-100	RayBiotech, Inc.	Rabbit anti-Human PAI-1	Rabbit Anti-Human PAI-1 antibody detects a target derived from a human recombinant PAI-1 protein aswell as selected cell lysates. Cross reactivity with mouse and rat PAI-1 was not determined.	ELISA (recommended work dilution= 1: 20,000)
MD-05-0135	RayBiotech, Inc.	Mouse anti-E. coli Shigatoxin (STX) 1 & 2		Suitable for use in ELISA (capture antibody at 20&micro,g/ml), Western blot (1&micro,g/ml) and Toxin Neutralization. Neutralizes Vero Cell toxicity of E. coli O157:H7 extracts when used at a 5 &micro,g/ml concentration. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
RB-01-0001-100	RayBiotech, Inc.	Mouse anti-Human bFGF/FGF basic/FGF2		ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
RB-01-0006-100	RayBiotech, Inc.	Mouse anti-Human TNF alpha	Human TNFalpha monoclonal antibody detects protein derived from human, Jurkat cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.	Direct ELISA: antibody detection limit dilution 1:10,000-20,000, Recommended for use at 5-10 ng/ml., Western Blot: Approx 26 and 17 kDa band observed in Jurkat cell lysate. this antibody can be used at 1 - 2 ?g/mL with the appropriate secondary reagents to detect human TNF-alpha.,
RB-08-0004-100	RayBiotech, Inc.	Rabbit anti-Human Apelin ligand (N-terminus)	Human Apelin polyclonal antibody detects protein derived from human cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.	(under testing)Direct ELISA: antibody detection limit dilution will be 1:10,000-20,000. Western Blot: This antibody will be used at 1 - 2 �g/mL with the appropriate secondary reagents to detect human Apelin. Immunohistochemistry: under testing
RB-08-0007-100	RayBiotech, Inc.	Rabbit anti-Human Amylin	Human Amylin polyclonal antibody detects protein derived from human cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.	ELISA (recommended work dilution= 1: 5,000), Western Blotting (recommended work dilution= 1: 1,000)
RB-IP-10-101-100	RayBiotech, Inc.	Mouse anti-HA-Tag Antibody	HA- Tag antibody detects fusion recombinant proteins derived from a variety of sources at various concentrations. No Cross reactivity with cell lysates was not found in ELISA and Western Blot.	ELISA (recommended work dilution= 1:1000-5000), Western Blotting( recommended work dilution= 1-2 �g/ml)
188-10073-1	RayBiotech, Inc.	EGF Receptor Antibody, Mouse Monoclonal Antibody	Human. Does not react with mouse and rat. Other species not known.	Functional Activity (Inhibition): antibody blocks EGF receptor activation, inhibits A431 cell proliferation, arrests tumor growth in vivo, affinity purification<br/>IP: 1-2�g/500ug protein lysate<br/>FACS: 0.5-1ug/million cells
188-10073-SAF	RayBiotech, Inc.	EGF Receptor Antibody [Sodium Azide Free], Mouse Monoclonal Antibody	Human. Does not react with mouse and rat. Other species not known.	Functional Activity (Inhibition): antibody blocks EGF receptor activation, inhibits A431 cell proliferation, arrests tumor growth in vivo, affinity purification<br/>IP: 1-2�g/500ug protein lysate<br/>FACS: 0.5-1ug/million cells
188-10772-1	RayBiotech, Inc.	Mouse anti-Human/Monkey CD3e Antibody, Mouse Monoclonal Antibody	Human and Monkey, Other species not tested.	Functional Activity: Induce T cell activation and proliferation (Order SAF formulation)<br/>Flow Cytometry: 0.5-1ug/million cells in 0.1ml<br/>Immunofluorescence: 1-2ug/ml
188-10772-SAF	RayBiotech, Inc.	Mouse anti-Human/Monkey CD3e Antibody [Sodium Azide Free], Mouse Monoclonal Antibody	Human and Monkey, Other species not tested.	Functional Activity: Induce T cell activation and proliferation (Order SAF formulation)<br/>Flow Cytometry: 0.5-1ug/million cells in 0.1ml<br/>Immunofluorescence: 1-2ug/ml
RB-01-0001B-50	RayBiotech, Inc.	Mouse anti-bFGF/FGF basic/FGF2 [+Biotin], Mouse Monoclonal Antibody		ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
RB-01-0006B-50	RayBiotech, Inc.	Mouse anti-TNF alpha [+Biotin], Mouse Monoclonal Antibody	Human TNFalpha monoclonal antibody detects protein derived from human, Jurkat cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.	Direct ELISA: antibody detection limit dilution 1:10,000-20,000, Recommended for use at 5-10 �g/ml., Western Blot: Approx 26 and 17 kDa band observed in Jurkat cell lysate. this antibody can be used at 1 - 2 �g/ml with the appropriate secondary reagents to detect human TNF-alpha.,
RB-IP-10-101B-50	RayBiotech, Inc.	Mouse anti-HA-Tag [+Biotin], Mouse Monoclonal Antibody	HA- Tag antibody detects fusion recombinant proteins derived from a variety of sources at various concentrations. No Cross reactivity with cell lysates was not found in ELISA and Western Blot.	ELISA (recommended work dilution= 1:1000-5000), Western Blotting( recommended work dilution= 1-2 �g/ml)
128-10210-1	RayBiotech, Inc.	B-Cell Leukemia/Lymphoma 2, Mouse Monoclonal Antibody		BCL2 antibody has been tested by ELISA, Western blot, and Immunofluorescence analyses to assure specificity and reactivity. Since applications vary, however, each investigation should be titrated by a reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500.<br/>Recommended starting dilution is 1:500.
128-10210-2	RayBiotech, Inc.	B-Cell Leukemia/Lymphoma 2, Mouse Monoclonal Antibody		BCL2 antibody has been tested by ELISA, Western blot, and Immunofluorescence analyses to assure specificity and reactivity. Since applications vary, however, each investigation should be titrated by a reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500.<br/>Recommended starting dilution is 1:500.
128-10210-3	RayBiotech, Inc.	B-Cell Leukemia/Lymphoma 2, Mouse Monoclonal Antibody		BCL2 antibody has been tested by ELISA, Western blot, and Immunofluorescence analyses to assure specificity and reactivity. Since applications vary, however, each investigation should be titrated by a reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500.<br/>Recommended starting dilution is 1:500.
128-10239-1	RayBiotech, Inc.	Killer Cell Immunoglobulin-Like Receptor 2 Domains Long Cytoplasmic Tail 3, Mouse Monoclonal Antibody		KIR2DL3 antibody has been tested by ELISA and Western blot immunofluorescent staining with flow cytometric analysis to assure specificity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Applications include flow cytometry (5-10g/1X?1000000), ELISA (1:1,000 when tested against the immunized protein) and western blot analysis (1&micro,g/ml).
128-10239-2	RayBiotech, Inc.	Killer Cell Immunoglobulin-Like Receptor 2 Domains Long Cytoplasmic Tail 3, Mouse Monoclonal Antibody		KIR2DL3 antibody has been tested by ELISA and Western blot immunofluorescent staining with flow cytometric analysis to assure specificity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Applications include flow cytometry (5-10g/1X?1000000), ELISA (1:1,000 when tested against the immunized protein) and western blot analysis (1&micro,g/ml).
128-10239-3	RayBiotech, Inc.	Killer Cell Immunoglobulin-Like Receptor 2 Domains Long Cytoplasmic Tail 3, Mouse Monoclonal Antibody		KIR2DL3 antibody has been tested by ELISA and Western blot immunofluorescent staining with flow cytometric analysis to assure specificity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Applications include flow cytometry (5-10g/1X?1000000), ELISA (1:1,000 when tested against the immunized protein) and western blot analysis (1&micro,g/ml).
128-10243-1	RayBiotech, Inc.	Killer Cell Immunoglobulin-Like Receptor 2 Domains Short Cytoplasmic Tail 4, Mouse Monoclonal Antibody		KIR2DS4 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000.
128-10243-2	RayBiotech, Inc.	Killer Cell Immunoglobulin-Like Receptor 2 Domains Short Cytoplasmic Tail 4, Mouse Monoclonal Antibody		KIR2DS4 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000.
128-10243-3	RayBiotech, Inc.	Killer Cell Immunoglobulin-Like Receptor 2 Domains Short Cytoplasmic Tail 4, Mouse Monoclonal Antibody		KIR2DS4 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000.
128-10257-1	RayBiotech, Inc.	Killer Cell Immunoglobulin-Like Receptor 2 Domains Long Cytoplasmic Tail 1, Mouse Monoclonal Antibody		KIR2DL1 antibody has been tested by ELISA, Western blot and immunoprecipitation analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:500.