| Applications: | SDS-PAGE |
| Species Reactivity: | Human |
| MW: | 28 kDa (246 aa), confirmed by MALDI-TOF. |
| Purity: | > 90% by SDS - PAGE |
| Description: | The 14-3-3 family plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 beta, a subtype of the 14-3-3 proteins, was found in B Cells, brain and liver etc. This 14-3-3 beta has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Recombinant human 14-3-3 beta was expressed in E. coli and purified by using conventional chromatography techniques. |
| Further Information: | Sequence: MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY KNVVGARRSS WRVISSIEQK TERNEKKQQM GKEYREKIEA ELQDICNDVL ELLDKYLIPN ATQPESKVFY LKMKGDYFRY LSEVASGDNK QTTVSNSQQA YQEAFEISKK EMQPTHPIRL GLALNFSVFY YEILNSPEKA CSLAKTAFDE AIAELDTLNE ESYKDSTLIM QLLRDNLTLW TSENQGDEGD AGEGEN |
| Synonym: | Tyrosine 3-monocxygenase/tryptopan 5-monooxygenase activation protein beta polypeptide, Tyrosine 3-monocxygenase/tryptopan 5-monooxygenase activation protein, beta polypeptide, 14-3-3 beta, YWHAB, GW128, HS1, KCIP-1, Tyrosine 3-monocxygenase/tryptopan 5-monooxygenase activation protein, beta polypeptide 14 3 3 protein beta/alpha, Brain protein 14 3 3 beta isoform, GW 128, HS 1, KCIP 1, KCIP1, Protein 1054, Protein kinase C inhibitor protein 1, Tyrosine 3 Monooxygenase/Tryptophan 5 Monooxygenase Activation Protein Beta Polypeptide. |
| Ascension Number: | P31946 |
| NCBI EntrezGene: | NP_003395 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
