HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY E2F4 (RC207699, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-E2F4 (1:500). Positive lysates LY400717 (100 ug) and LC400717 (20 ug) can be purchased separately from OriGene.