HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY EEF1D (Cat# RC224856, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EEF1D (Cat# TA811677)(1:2000). Positive lysates [LY429804] (100 ug) and [LC429804] (20 ug) can be purchased separately from OriGene.