HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ECE1 (Cat# RC223135, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ECE1(Cat# TA804823). Positive lysates [LY419958] (100 ug) and [LC419958] (20 ug) can be purchased separately from OriGene.