HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NRAS (Cat# RC202681, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NRAS(Cat# TA505835). Positive lysates [LY400901] (100 ug) and [LC400901] (20 ug) can be purchased separately from OriGene.