HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NRIP3 (Cat# RC202937, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NRIP3(Cat# TA504936). Positive lysates [LY412407] (100 ug) and [LC412407] (20 ug) can be purchased separately from OriGene.